Evaluation of Some Intestinal Biomarkers in the Determination of Intestinal Damage in Calves with Coccidiosis

This study aimed to assess the usefulness of injury biomarkers specific intestines in identifying presence and degree intestinal epithelial damage coccidiosis-infected calves. Forty calves various breeds sexes, aged 21 days 60 days, were used study. Of these, 30 experimental group, 10 healthy control. The McMaster Oocyte counting technique was diagnose Eimeria confirm clinical coccidiosis. Cases with signs more than 5,000 oocysts gram feces included All had blood samples drawn at 0 th hours 72 nd hours. Blood gas measurements performed a analyzer. Hemogram an automated hematologic Bovine-specific enzyme-linked immunosorbent analysis (ELISA) test kits measure fatty acid binding protein (I-FABP), trefoil factor-3 (TFF-3), alkaline phosphatase (IAP), claudin-3 (CLD-3), smooth muscle actin (ACTG2), interleukin-8 (IL-8) from serum samples. Calves coccidiosis received single dose toltrazuril (15 mg/kg) supportive care. Before treatment (0 hours) calves, I-FABP CLD-3 levels greater (p<0.05), after (72 hours), TFF-3 ACTG2 higher There significant decrease IL-8 compared pre-treatment (p<0.05). I-FABP, TFF-3, CLD-3, ACTG2, are helpful reliable that can be utilized epithelium